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The volume of pre-chilled PBS added to each brain sample was calculated based on the estimated protein content (~5% of the fresh tissue weight) to reach a 20 g/L concentration

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However, this observation is somewhat difficult to reconcile with modelled data (Polle 2001)

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Similarly, bariatric procedures such as Roux-en-Y gastric bypass alter the anatomy and mixing of digestive contents, leading to impaired B12 absorption and necessitating lifelong B12 supplementation according to American Society for Metabolic and Bariatric Surgery guidelines

Each method has specific timing requirements and lifestyle considerations