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Free GTP that colocalized in the mitochondria declined in old age Since GTP is primarily produced in mitochondria by the catalytic function of succinyl-CoA synthetase in the TCA cycle, we explored the presence of free GTP pools in the mitochondria in hippocampal neurons, by probing for colocalization with the membrane potential dye TMRM, whose positive charge favors distribution into negatively polarized mitochondria [23]

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