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PLoS One 5 , e9582 (2010)

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C-peptide levels are measured instead of insulin levels because C-peptide can assess a person's own insulin secretion even if they receive insulin injections, and because the liver metabolizes a large and variable amount of insulin secreted into the portal vein but does not metabolize C-peptide, meaning blood C-peptide may be a better measure of portal insulin secretion than insulin itself

Fungi, on the other hand, can produce significant amounts of protein (ranging from 20% to 85%, dwb.) depending on the genus, species, and culture conditions (Bakratsas et al., 2021

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Thus, mouse hepatocytes used in this study have much strength and can be used for investigating the link between GSH-deficiency, oxidative stress, and VD regulatory genes