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The systemic effect observed in mice was then confirmed in cultured human heart cells
Several factors may hinder the absorption of natural food folate, e.g., partial release from the food matrix (incomplete liberation from cellular structures), destruction within the gastrointestinal tract, and incomplete hydrolysis of polyglutamates to monoglutamates (possibly mediated by partial inhibition of deconjugation enzymes by other dietary constituents such as organic acids)
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The potential role of gut microbiome composition is a theoretical consideration that has not yet been substantiated by robust clinical evidence in this context
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