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So the whole protocol ends up being an hour long

In this regard, we evaluated possible fluorescence quenching upon mixing PLGA nanoparticles encapsulating DDA-GSH-AuNCs with cell growth media, which typically contain various ions, small molecules and proteins

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The experiment was performed in the presence and absence of CellTox Green Dye to determine if the fluorescent dye had an effect on the subsequent luminescent signal of the GSH/GSSG-Glo Assay