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As a result of the study, it was observed that the administered melatonin had a beneficial effect on the lipid profile, the percentage of fat present in the abdominal cavity, the body weight of the rats, and also the overall insulin concentration in the body

It's a one-two powerful punch of support for the immune system, boosted even further with the addition of zinc, selenium, vitamin C and vitamin D3

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PMID:17690

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After washing, sections were incubated 30 min with horseradish peroxidase-labelled goat-anti-rabbit (GARPO) or rabbit-anti-goat (RAGPO) secondary antibodies diluted in 1% BSA/PBS + 1% normal human (AB) serum to stop unspecific binding