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Footnotes # Co-senior authors Abbreviations BRET Bioluminescence resonance energy transfer BSA Bovine serum albumin CyOFP Cyan-excitable orange fluorescent protein DERET Diffusion-enhanced resonance energy transfer FCS Fluorescence correlation spectroscopy FITC Fluorescein isothiocyanate GFP Green fluorescent protein GLP-1(R)(A) Glucagon-like peptide-1 (receptor) (agonist) GUV Giant unilameller vesicle MCD Methyl--cyclodextrin Nb37 Nanobody-37 POPC 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine T2D Type 2 diabetes TMR Tetramethylrhodamine TR-FRET Time-resolved Frster resonance energy transfer

Large-scale purification was conducted and the peptide 20GIPR/GLP-1RG s complexes were collected by SEC for cryo-EM studies, with all components of the complex identified in SDS-PAGE of the SEC peak (Supplementary Fig

This mixed neuroblastic and melanocytic phenotype is also demonstrated at the ultrastructural level

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1 2 "Diagnosing vitamin B-12 deficiency on the basis of serum B-12 assay"

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