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RNA-seq RNA was isolated using Direct-zol RNA micro prep (Zymo, UK) as per the manufacturers protocol

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Emerging evidence indicates that vitamin D, through receptor-mediated signaling, participates in multiple pathophysiological processes, including the regulation of immune and inflammatory responses, the mitigation of oxidative stress, the preservation of mitochondrial function, the inhibition of renal fibrosis, modulation of the reninangiotensinaldosterone system (RAAS), and the maintenance of gutkidney axis homeostasis

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Hippuric acid, a glycine conjugate involved in liver detoxification, showed a decrease post-seizure, likely indicating a depletion of glycine, which also functions as a neurotransmitter in inhibitory signalling in the central nervous system 33

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