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Brain Res 617: 285295, 1993
For IRE1 cleavage assay, purified RNA substrates were first incubated with inhibitor or vehicle (DMSO) for 30 min on ice, then incubated with recombinant GST-tagged human IRE1 (aa 465-977 corresponding to the kinase/ribonuclease domain, 11905-H20B, Sino Biological) in reaction buffer (40 mM HEPES pH 7.0, 10 mM Mg(OAc) 2 , 50 mM KOAc, 5 mM DTT, 1 M ADP) for 30 minutes at 30 C
[DOI] [PubMed] [Google Scholar] 102.Engelhard Y.N., Gazer B., Paran E
A PubMed-indexed fibroblast culture study reported that GHK-Cu stimulated collagen synthesis in fibroblast cultures
Anal Biochem 106:207212 Guo J, Zhou R, Ren X, Jia H, Hua L, Xu H, Lv X, Zhao J, Wei T (2018) Effects of salicylic acid, Epi-brassinolide and calcium on stress alleviation and Cd accumulation in tomato plants
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