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The fixed ratio of the 80 mg vial keeps the inter-peptide variables constant across animals in a cohort, which makes between-subject comparisons cleaner than asking each researcher to reconstitute four separate vials

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RNA isolation and real-time quantitative PCR Total RNA was isolated with E.Z.N.A. Total RNA Kit I (Omega Cat#R6834-01) and reverse transcription (RT) reactions were performed with High-Capacity cDNA Reverse Transcription Kit (ThermoFisher Cat#4368814) according to manufacturers protocol

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Notably, BUD13 immunoprecipitated WT F-SNIP1 more efficiently than the F-SNIP1 mutants, indicating a reduced ability of the mutants to associate with the activated spliceosome (Fig

Link van Groeningen, C