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LaSalle JR, Berria R

Diabetes 63 , 33463358 (2014)

Recommended Timing Protocols Morning Fasted Protocol (Most Common): Timing: Upon waking, before any food or caloric beverages Fasting Window: At least 8-10 hours fasted from overnight sleep Post-Injection: Wait 30-60 minutes before eating breakfast Activity: Light fasted cardio or morning routine before first meal may enhance fat mobilization Advantages: Convenient, consistent daily routine, maximizes overnight fasted state Pre-Exercise Protocol: Timing: 30-45 minutes before fasted cardio or training session Fasting Window: At least 4-6 hours since last meal Rationale: Exercise during fasted state with elevated free fatty acids may enhance fat oxidation Considerations: Not suitable for high-intensity training

Paul Jarrod Frank, a cosmetic dermatologist, in the New York Times

RAW 264.7 cells were cultured in complete Dulbeccos modified Eagle medium (DMEM, Gibco) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (FBS) (Avantor Seradigm), 2 mM L-glutamine (Gibco), 1 non-essential amino acids solution (Gibco) and 1 mM sodium pyruvate (Gibco) below 18 passages without exceeding 90% confluency

Reuss, DE