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Injections and IV infusions, meanwhile, deliver precise, high systemic levels under professional supervision

For experiments involving oxygen control, purified CD8 + T cells were immediately acclimatized to the required oxygen tension (21%, 5% or 1% O 2 ) for 2 h in complete RPMI 1640 supplemented with 10% FBS, 100 U ml 1 penicillin and 100 g ml 1 streptomycin, before activation with anti-CD3/CD28 Dynabeads and 30 U ml 1 IL-2

They were encouraged by the fact glucagon was proven to be the most potent substance then known to increase the secretion of insulin

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This population-specific safety evidence is more relevant for clinical application than data from exclusively healthy volunteers would be