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Cancer Res (Suppl)

After the glycosylation, the affinity support, the protein is bound to unbound contaminants are washed away, and the Eluted protein was purified

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Dihexa solutions were prepared by suspending dry stock dihexa in DMSO at 3 rag/niL and subsequent serial dilutions in 50% DMSO or HPLC grade water for the final the proteins present in the plasma samples were precipitated using three volumes of ice-cold acetonitrile

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These approaches provide quantitative metrics to support tumor staging, prognostic evaluation, and monitoring of treatment response