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Abbreviations ELISA: Enzyme-linked immunosorbent assay IgG: Immunoglobulin G HRP: Horseradish peroxidase enzyme label O.D.: Optical density PBS: Phosphate buffer saline FBS: Fetal bovine serum Ab-c: Capturing antibody Ab-d: Detection antibody References Karen L, Viswanath D, Aidas K et al (2004) (2012, updated in 2019) Assay Guidance Manual

Known scientifically as Alanine-Histidine-Lysine copper, this specialized tripeptide complex is frequently studied for its unique cellular interactions

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The oncometabolite D-2HG produced in IDH-mutant tumors from KG contributes to inhibiting antitumor immunity via the metabolic reprogramming of CD8+ T cells and decreasing their proliferation and antitumor toxicity [100]