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c) condensing Fmoc-Thr(tBu)-Phe-Thr(tBu)-Ser(tBu)-Asp(OtBu)-Val-Ser(Oxa)-OH (fragment-2) with peptide obtained in step-b) in presence of a coupling agent in in-situ manner followed by deprotection in presence of base to obtain H-Thr(tBu)-Phe-Thr(tBu)- Ser(tBu)-Asp(OtBu)-Val-Ser(Oxa)-Ser(tBu)-Tyr(tBu)-Leu-Glu(OtBu)-Gly-Gln(Trt)- Ala-Ala-Lys(C18diacidmono-t-butyl-Y-Glu(AEEA-AEEA)-OtBu)-Glu(OtBu)-Phe-Ile- Ala-Trp(B oc) -Leu -V al- Arg(pbf) -Gly- Arg (pbf) -Gly-OtBu
Avoid the front of the arm (biceps area), the deltoid, and any area close to the elbow joint
Belknap, S., Blalock, E