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All blots were blocked for 30 minutes in 5% milk (dissolved in 1 TBST, TBST: 20 mM Tris pH 7.5, 150 mM NaCl, 0.1% Tween-20) at room temperature and incubated in primary antibody overnight at 4 o C

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Elevated postoperative endogenous GLP-1 levels mediate effects of roux-en-Y gastric bypass on neural responsivity to food cues