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Freezing reconstituted solutions is generally discouraged because it can lead to aggregation and loss of activity due to ice crystal formation, especially if the solution isn't specifically formulated with cryoprotectants

Declaration of Interest None of the authors of this paper have any real or apparent conflicts of interest to disclose, nor have any financial or consulting relationships to report

This ensures accurate concentration and consistent dosing across the vial's lifespan

This larger volume can make it easier to measure tiny doses accurately

It was first isolated from human plasma, where it naturally occurs at concentrations of around 200 ng/mL at age 20, declining to approximately 80 ng/mL by age 60

Reference: Gwyer D, Wragg NM, Wilson SL (2019)