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Agrobacterium cells were collected by centrifugation, resuspended in infiltration buffer (10 mM MgCl, 200 M acetosyringone, 10 mM MES) to an OD of 0.70.8, and incubated at room temperature for 2 h

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The mechanisms underlying IGF-1's tendon repair effects involve activation of the IGF-1 receptor on tenocytes and tendon stem cells, leading to enhanced extracellular matrix synthesis, improved cellular migration to injury sites, and optimized remodeling of repair tissue

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