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Finally, transfected neurons were immunostained for presynaptic markers, VGLUT1 and synapsin, and postsynaptic marker, PSD-95, to assess functional synapse formation

Precursor ion mass spectra were acquired at 120,000 resolution, scan range 375-1,500 and maximum injection time 50 ms

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Others advise against it due to limited safety data on the combination

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10.1038/nature00965 Nature 74 RidderstraleM.DegermanE.TornqvistH