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The insoluble portion was subsequently removed by centrifugation and the supernatant was incubated with Ni-NTA resin (GE Healthcare) for 2 h batch binding at 4 C

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The study confirmed that brain homogenates could also be effectively used to evaluate DSIPs influence on mitochondrial functional state, with DSIP and Deltaran showing comparable activities in both isolated mitochondria and brain homogenates

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