After oral treatment with betanin, they found that followed by a significant increase of glycolytic enzyme (glucokinase and pyruvate kinase), glucose6phosphate dehydrogenase, and decrease in the activity of gluconeogenic enzymes (glucose6phosphatase and fructose1,6bisphosphatase), the levels of plasma glucose, insulin, glycosylated hemoglobin (HbA1c), and hemoglobin (Hb) were restored in diabetic rat
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Curr Heart Fail Rep 9:164173
Then, the same co-first author or another co-first author randomly (in the same way as above) picked the proper amount (depending on the first round of statistics) of the tif image for image measurement and data entry